Preparation of agar wells for antibiotic assay.

A variety of devices such as stainless-steel or ceramic cylinders, single or multiple in design, and paper discs of several sizes are commonly used for antibiotic assays performed by the agar diffusion method. Holes cut in agar (agar wells) are wells with melted agar is often necessary to obtain uniform inhibition zones (2). This note describes a method for the preparation of agar-well plates which obviates the disadvantages. In our laboratory, agar-well assays